Minimum Information about a Flow Cytometry Experiment

MIFlowCyt 1.0

A standard for outlining the minimum information required to report the experimental details of flow cytometry experiments

Original Article

Document Status

MIFlowCyt is an ISAC Recommendation. This document has been reviewed by members of the International Society for Analytical Cytology (ISAC) and other interested parties and has been endorsed by the ISAC President and ISAC Council as an ISAC Recommendation. It is a stable document and may be used as reference material. This document has been produced by the ISAC Data Standards Task Force (ISAC DSTF) and other members of scientific community as acknowledged below.

The International Society for Analytical Cytology (ISAC,
The Flow Informatics and Computational Cytometry Society (
The Bioinformatics Standards for Flow Cytometry Consortium (

MIFlowCyt development has been supported by NIH grant EB005034 from the National Institute of Biomedical Imaging and Bioengineering and by the NIAID Bioinformatics Integration Support Contract AI40076 (BISC). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Biomedical Imaging and Bioengineering or the National Institutes of Health. Special thanks to James Wood for chairing the ISAC adoption process.


The fundamental tenet of scientific research is that the published results of any study have to be open to independent validation or refutation. The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) establishes criteria for recording and reporting information about the flow cytometry experiment overview, samples, instrumentation and data analysis. It promotes consistent annotation of clinical, biological and technical issues surrounding a flow cytometry experiment by specifying the requirements for data content and by providing a structured framework for capturing information.


Throughout the biological and biomedical sciences prescriptive checklists specifying the key information to include when reporting experimental results are beginning to find favour with experimentalists, analysts, publishers and funders alike. In order to simplify cross-domain comparison and integration, MIFlowCyt has been developed in a coordinated manner with checklists for other high-throughput experiments that are joined within the Minimum Information for Biological and Biomedical Investigations (MIBBI) project.

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Last update: February 26th, 2008